In this investigation,48 live specimens of the genus spermophilus Cuvier,1825 colected from the type localities within various of Iran have been sudied.The specimens were studied in detail with respect to their kariological characteristics. 

The karyotypic analysis of spermophilus fulvus Lichtenstein ,1823 from Iran showed that its diploid chromosome number is 2n=36 the Xchoromosome is metacentric and medium in size and the Ychromosome is the smallest complement of the karyotype seem to be acricentric and the autosomes contain seven pairs of large to medium - sized subtelocentric.Four pairs of large to medium - sized subtelocentric.Eight pairs of large to small acrocentric and one pair of small metacentric.

A comparative study was carrid out between the karyotype data obtainend from these species and the data from the literature.