Apics mellifera meda in one among those two dozen subspecies which are the most widely distributed in the world . While in our first description , its distribution is restricted to North persia, lencoron (on the Caspian sea coast),in later staudies it extent westward, to the north of iraq and syria, and to the southeaste parts of turkey .To investigate the geografic variation and population structure of the honey bees of northen Iran that are among the least studied populations of Apis mellifera,samples were collected in the Alburz mountains,in the south of the Caspian sea.

Atotal of 46 colonies were sample from six different locations (    three in the highlands:khanchay,sefra,zanjan;and three in the lowlands,Gholikandy,Gultepe and duzteran fig.1).outof six enzymes assayed, four were found to be polymorphic( Pgm,Hk, Mdh and Est)  and two Pgi and Me displayed invariant banding pattes.

Gene frequencies,enzyme heterozygosities and usefulness of gene  frequencies to Hardy-weinberg expectations were analyzed with the BIOSYS-1 package.the overall grand mean heterozygosity for all populations was calculated as 0.052-+0.036.Adistance wagner analysis based on the prevosti distance divided the honey bee samples into two groups according to their origin from lowlands and highlands. Morphmetric variations of the colonies were assessed using parameters measured from hind and fore-wing and from legs.Different multivariate analyses were applied for the determination of different honey bee population in the Alburz Mountains in Iran .